What's on the PBT
| Area | Share |
|---|---|
| Specimen Collection | 45–50% |
| Specimen Handling, Transport and Processing | 15–20% |
| Laboratory Operations | 15–20% |
| Circulatory System | 5–10% |
| Waived and Point of Care Testing | 5–10% |
| Non-Blood Specimens | 5–10% |
From ASCP's content guideline revised September 25, 2025. Testing is at Pearson VUE centers.
Eligibility routes
- Route 1: a NAACLS-approved program
- Route 2: 40 classroom hours, 100 clinical hours and 100 successful unaided venipunctures
- Route 3: 1 year full-time experience
- Route 4: RN, LPN or allied health education with phlebotomy training and 100 successful unaided collections
- Routes 5 to 7: current MLS or MLT (ASCP), DPT (ASCP), or a CDPH-approved California program
Know you're ready for the PBT (ASCP) before you book your date
PassPulse is built to get you through the PBT (ASCP) on your first attempt. Exam style questions on every topic, every answer explained, and a readiness score that tells you when you're ready. 15 minutes a day, on your phone.
Start preparing for the PBT (ASCP)Retakes and renewal
You can attempt the exam 5 times under one eligibility route; after that you need to qualify under a different route. Each attempt needs a new application fee. The credential is maintained through ASCP's Credential Maintenance Program: 9 points every 3 years and a $110 fee.
PBT (ASCP) FAQ
What is the passing score for the ASCP phlebotomy exam?
400 on a scale from 100 to 999.
How many questions are on the ASCP PBT?
80 questions in 2 hours.
How much is the ASCP phlebotomy exam?
$155 application fee, non-refundable.