Free CRCST practice · Updated October 2026

CRCST high-level disinfection and endoscopes practice questions, with every answer explained

Cleaning, Decontamination and Disinfection is 21% of the scored CRCST exam. Answer 10 exam style questions on high-level disinfection and endoscopes and see exactly which concepts would cost you points on test day.

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Where the CRCST points areshare of 125 scored items
Cleaning, Decontamination and Disinfection
21%
Preparation and Packaging
21%
Sterilization Process
21%
Departmental Considerations
15%
Sterile Storage, Transport and Inventory Management
9%
Professional Development and Human Relation Skills
8%
Patient Care Equipment and Distribution
5%
Source: CRCST exam content outline (HSPA)You are here ↑
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Q1Minimum effective concentration

Before placing a bronchoscope into a reusable high-level disinfectant basin, the technician dips a chemical test strip into the solution. What is the strip checking?

What these high-level disinfection and endoscopes questions test

These questions cover minimum effective concentration, shelf life vs use life, solution management, glutaraldehyde safety and OPA. Each one shows the correct answer and why every other option is wrong, so a miss tells you exactly what to review before the CRCST.

Minimum effective concentrationShelf life vs use lifeSolution managementGlutaraldehyde safetyOPAGlutaraldehyde activationEndoscope pre-cleaningLeak testingEndoscope reprocessing sequenceEndoscope storage

All 10 questions with answers explained

Prefer to read first? Every question from the practice set above, with the correct answer and why each wrong option is wrong.

Q1Before placing a bronchoscope into a reusable high-level disinfectant basin, the technician dips a chemical test strip into the solution. What is the strip checking?
  1. That the solution temperature is within its labeled range
  2. That the bronchoscope is free of protein soil
  3. That the active ingredient is at or above its MEC
  4. That the solution pH is neutral and safe for skin

Answer: C. Reusable HLD solutions are diluted by water carried in on devices and weaken with use and time. The test strip verifies the active ingredient is at or above its minimum effective concentration (MEC) before the solution is used, and the result is documented.

Why not A: Temperature is checked with a thermometer or the system display, not with a concentration strip.

Why not B: Cleaning verification tests check for residual soil on devices. The MEC strip tests the solution.

Why not D: The strip does not measure skin safety or pH. It confirms the germicide concentration.

Exam tactic: MEC strip = is the soup still strong enough.

Q2The expiration date printed on an unopened jug of high-level disinfectant in the storeroom refers to which of the following?
  1. The reuse life of the solution once it is poured into a basin
  2. The number of scopes the solution can process before testing
  3. The date the test strips for that product will expire
  4. The shelf life of the product in its sealed container

Answer: D. Shelf life is how long the product remains stable in its unopened original container. Once the product is activated or poured for use, a separate use life or reuse life begins, and the solution is discarded at whichever limit comes first.

Why not A: Reuse life starts when the solution is activated or put into use. It is not the date on the sealed jug.

Why not B: The jug date is not a count of devices. Concentration is checked with MEC strips before use.

Why not C: Test strips have their own expiration date on their own container.

Exam tactic: Shelf life on the shelf, reuse life in the basin.

Q3The level in a manual HLD soaking basin is low, and a coworker suggests pouring in fresh solution from a new jug to fill it back up. Why is this wrong?
  1. Fresh solution is too strong and will damage endoscopes
  2. The basin must stay half full so scopes can be coiled
  3. Topping off mixes new and used solution, voiding tracking
  4. New solution must be heated before it is ever added

Answer: C. Adding fresh solution to used solution creates a mixture with no valid activation date or reuse life and can mask a weakened solution. When the volume is too low to immerse devices, the basin is emptied, cleaned per the IFU, and refilled with fresh solution.

Why not A: Fresh solution at labeled strength is what devices are validated for. The issue is mixing, not strength.

Why not B: Devices must be completely immersed, so a low basin is a problem that topping off does not properly solve.

Why not D: Temperature requirements come from the label. The core error here is mixing new solution into old.

Exam tactic: Never top off: dump, clean, refill.

Q4Staff in an endoscopy reprocessing room report eye and nose irritation and headaches while using glutaraldehyde. Which control most directly addresses the cause?
  1. Switching to latex gloves for all reprocessing
  2. Keeping basins covered and using local exhaust ventilation
  3. Raising the solution temperature to speed evaporation
  4. Leaving soaking basins open so vapors spread out

Answer: B. Glutaraldehyde vapor irritates the eyes, nose, throat, and lungs and can cause sensitization. Keeping containers closed with tight lids, using local exhaust or a ventilated hood, and good room ventilation keep vapor levels low, along with proper PPE.

Why not A: Glove type does not address vapor inhalation, and latex is not the recommended barrier for glutaraldehyde.

Why not C: Warmer solution releases more vapor and increases exposure.

Why not D: Open basins release more vapor into the breathing zone and worsen symptoms.

Exam tactic: Lid on, exhaust on.

Q5A technician notices gray stains on her forearm after handling ortho-phthalaldehyde (OPA) without full sleeve protection. What does this indicate?
  1. The solution has dropped below its minimum effective concentration
  2. The solution was contaminated by tap water during filling
  3. OPA stains proteins, including skin, so skin contact occurred
  4. OPA turns gray only when it has passed its reuse life

Answer: C. OPA reacts with proteins and stains them gray, including unprotected skin, mucous membranes, and clothing. The staining shows a PPE gap, so gloves, fluid-resistant sleeves or gowns, and eye protection must be used when handling it.

Why not A: Concentration is checked with a test strip. Skin staining is a sign of exposure, not of a weak solution.

Why not B: Dilution affects concentration, not whether OPA stains skin.

Why not D: OPA stains proteins whether it is fresh or near the end of its reuse life.

Exam tactic: OPA paints proteins gray.

Q6A technician opens a new container of glutaraldehyde that comes with a separate activator vial. What must happen before it is used?
  1. Dilute it with tap water to half strength
  2. Add the activator, record the date, and test MEC
  3. Heat it in the washer until the activator dissolves
  4. Use it right away, since the activator is optional

Answer: B. Acidic glutaraldehyde solutions are activated with an alkaline activator to reach the pH where they are most biocidal. The activation date starts the use life, so it is recorded on the container and log, and the solution is tested with an MEC strip before use.

Why not A: Diluting a ready-to-use HLD lowers it below its labeled concentration.

Why not C: The activator is mixed in per the label, not heated in a washer.

Why not D: Without activation the solution is not in its labeled effective state, and the use life cannot be tracked.

Exam tactic: Activate, date it, test it.

Q7An upper GI endoscopy has just ended in the procedure room. What should happen to the endoscope immediately, before it is transported to reprocessing?
  1. Place it dry in a bag and send it when the room is turned over
  2. Coil it on the cart and wait until the end of the day schedule
  3. Wipe the outside and suction detergent through its channels
  4. Soak it in high-level disinfectant in the procedure room

Answer: C. Point-of-use pre-cleaning at the bedside, wiping the insertion tube and flushing or suctioning detergent solution through the channels, keeps soil from drying and helps prevent biofilm. The scope is then transported promptly in a closed, labeled container.

Why not A: Letting soil dry in the channels makes cleaning harder and promotes biofilm.

Why not B: Delays allow organic material to dry and harden inside channels.

Why not D: HLD is never applied to a scope that has not been leak tested and manually cleaned.

Exam tactic: Wipe and suction before the scope leaves the room.

Q8A flexible endoscope arrives in the reprocessing room after bedside pre-cleaning. When is the leak test performed?
  1. Before the scope is immersed for manual cleaning
  2. After high-level disinfection, before final rinsing
  3. After drying, just before the scope is hung for storage
  4. Only when the scope shows visible damage on inspection

Answer: A. The leak test is done before immersion and manual cleaning to find holes or damage in the scope's covering or channels. Immersing a leaking scope lets fluid enter the internal parts, causing major damage and a hidden contamination risk.

Why not B: By this point the scope has already been immersed several times, so a leak would have caused fluid invasion.

Why not C: Testing at the end misses leaks before fluid has entered the scope.

Why not D: Many leaks are not visible, so the test is done on every scope every time.

Exam tactic: Leak test before it gets wet.

Q9Which sequence correctly lists the main steps of flexible endoscope reprocessing?
  1. Leak test, HLD, manual cleaning, pre-clean, dry, rinse, store
  2. Pre-clean, leak test, manual clean, HLD, rinse, dry, store
  3. Manual clean, pre-clean, HLD, leak test, rinse, store, dry
  4. Pre-clean, HLD, leak test, rinse, manual clean, dry, store

Answer: B. Flexible endoscopes are pre-cleaned at the point of use, leak tested, manually cleaned, high-level disinfected or sterilized, rinsed, dried, and stored. Each step depends on the one before it, so cleaning always comes before disinfection.

Why not A: HLD before cleaning fails because soil protects microorganisms, and pre-cleaning belongs first.

Why not C: Pre-cleaning happens at the bedside before manual cleaning, and leak testing comes before immersion.

Why not D: HLD before leak testing and manual cleaning puts the disinfectant on soil and risks fluid invasion.

Exam tactic: Pre, leak, clean, kill, rinse, dry, hang.

Q10How should a flexible endoscope be stored after reprocessing and drying?
  1. Hanging vertically in a ventilated cabinet, valves removed
  2. Coiled tightly in its carrying case with valves attached
  3. Lying flat on an open shelf with the caps put back on
  4. Hanging in the decontamination room near the sinks

Answer: A. Hanging the scope vertically in a clean, ventilated cabinet lets the channels keep draining and drying. Valves and detachable parts are removed during storage, and the scope is protected from recontamination.

Why not B: Carrying cases are for transport. Coiled storage traps moisture and the case can harbor organisms.

Why not C: Lying flat with caps on traps moisture and leaves the scope exposed to contamination.

Why not D: The decontamination room is a dirty area, so clean scopes are never stored there.

Exam tactic: Hang it, vent it, pull the valves.

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