Free Phlebotomy practice · Updated October 2026

Phlebotomy infection control and PPE practice questions, with every answer explained

Safety and Compliance is 26 of the 100 scored items on the NHA CPT exam. Answer 10 exam style questions on infection control and PPE and see exactly which concepts would cost you points on test day.

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Where the NHA CPT points are100 scored items
Routine Blood Collections
28
Safety and Compliance
26
Patient Preparation
20
Processing
14
Special Collections
12
Source: NHA CPT test plan (2025)You are here ↑
Score 0/0
Q1Standard precautions

Standard precautions assume which of the following?

What these infection control and ppe questions test

These questions cover standard precautions, isolation, hand hygiene, glove removal and equipment hygiene. Each one shows the correct answer and why every other option is wrong, so a miss tells you exactly what to review before the NHA CPT.

Standard precautionsIsolationHand hygieneGlove removalEquipment hygieneRespiratorsInfection controlPPESpillsGloves

All 10 questions with answers explained

Prefer to read first? Every question from the practice set above, with the correct answer and why each wrong option is wrong.

Q1Standard precautions assume which of the following?
  1. Only patients with a known infection are a risk
  2. All blood and body fluids may be infectious
  3. Gloves are needed only for isolation patients
  4. Only visible blood is a risk

Answer: B. Standard precautions treat every patient's blood and most body fluids as potentially infectious, regardless of diagnosis.

Why not A: Unknown infections are common, so all patients are treated the same.

Why not C: Gloves are used for every venipuncture.

Why not D: Other body fluids can also carry pathogens.

Exam tactic: Treat all blood as infectious.

Q2A patient has suspected tuberculosis. What must the phlebotomist wear to enter the room?
  1. A surgical mask only
  2. A fit-tested N95 or higher respirator
  3. Gloves only
  4. A gown and gloves only, since tuberculosis spreads by contact with surfaces

Answer: B. Tuberculosis is airborne. Airborne precautions require a fit-tested N95 or higher respirator and an airborne infection isolation room.

Why not A: A surgical mask does not filter airborne particles well enough.

Why not C: Gloves alone do not protect against airborne spread.

Why not D: Tuberculosis spreads through the air, not mainly by contact.

Exam tactic: TB: fit-tested N95.

Q3When washing hands with soap and water, how long should the hands be scrubbed?
  1. About 5 seconds
  2. Exactly 2 minutes every time
  3. At least 20 seconds
  4. Until the soap is rinsed away

Answer: C. Hands are scrubbed with soap for at least 20 seconds, covering all surfaces including between fingers and under nails, before rinsing. CDC healthcare guidance sets a minimum of 15 seconds and accepts 20, which is the time most phlebotomy programs teach.

Why not A: Five seconds is too short to remove organisms.

Why not B: Two minutes is not the routine requirement.

Why not D: Rinsing is a separate step after scrubbing.

Exam tactic: Scrub 20 seconds.

Q4What is the correct way to remove contaminated gloves?
  1. Peel inside out, glove to glove, skin to skin
  2. Bite a fingertip to loosen each glove
  3. Pull both gloves off together by the fingertips at once
  4. Rinse them off and remove them later

Answer: A. The first glove is grasped at the palm and peeled off inside out, held in the gloved hand, then the second is removed by sliding a bare finger under its cuff, trapping the first inside. Hand hygiene follows.

Why not B: The mouth never touches contaminated gloves.

Why not C: Pulling by the fingertips can snap contamination onto skin.

Why not D: Gloves are removed, not rinsed and reused.

Exam tactic: Glove to glove, skin to skin.

Q5When should the phlebotomy chair armrest be disinfected?
  1. Never, it is not patient contact
  2. Between patients
  3. Once a month
  4. Only if blood is visible

Answer: B. Surfaces patients touch, such as armrests, are cleaned with an approved disinfectant between patients.

Why not A: Armrests are direct patient contact surfaces.

Why not C: Monthly cleaning allows spread between patients.

Why not D: Invisible contamination can still spread infection.

Exam tactic: Clean the armrest every patient.

Q6How often is fit testing generally required for an N95 respirator user?
  1. At least once a year
  2. Only once in a career
  3. Never, one size fits all
  4. Every day before use

Answer: A. OSHA requires fit testing before first use and at least annually, plus a user seal check each time the respirator is put on.

Why not B: A single test is not enough.

Why not C: Respirators come in sizes and must be fitted.

Why not D: Daily checks are seal checks, not fit tests.

Exam tactic: N95: fit test yearly, seal check every time.

Q7In the chain of infection, what does a patient with a weakened immune system represent?
  1. Susceptible host
  2. Reservoir
  3. Portal of exit
  4. Mode of transmission

Answer: A. A susceptible host is a person who can easily become infected, such as someone with a weakened immune system.

Why not B: The reservoir is where the organism lives and grows.

Why not C: The portal of exit is how an organism leaves a reservoir.

Why not D: Mode of transmission is how the organism travels.

Exam tactic: Weak immunity: susceptible host.

Q8Should a lab coat worn for blood collection be worn into the break room?
  1. No, it is removed before leaving the work area
  2. Yes, if it looks clean
  3. Yes, if it is buttoned
  4. Only on short breaks

Answer: A. Protective clothing is removed before leaving the work area so contamination is not spread to clean areas.

Why not B: Contamination is not always visible.

Why not C: Buttoning does not prevent spread.

Why not D: Break length does not matter.

Exam tactic: PPE stays in the work area.

Q9A small blood spill is on a counter. What is the correct approach?
  1. Wipe it with a dry paper towel and throw it in the regular trash
  2. Cover it and wait for housekeeping to arrive the next morning
  3. Spray it with water and let it air dry
  4. Wear gloves, absorb the blood, then clean and disinfect the area with an approved disinfectant

Answer: D. With gloves on, the spill is absorbed, the area is cleaned, and then it is disinfected with an approved disinfectant such as a 1:10 bleach solution or an EPA-registered product. Waste goes in biohazard containers as required.

Why not A: Wiping without gloves and disinfection is unsafe.

Why not B: Leaving a spill exposes others.

Why not C: Water does not disinfect.

Exam tactic: Gloves, absorb, clean, disinfect.

Q10According to OSHA, when must gloves be worn for blood collection?
  1. Only if the patient has a known infection
  2. Only for capillary punctures
  3. For every vascular access procedure, including venipuncture
  4. Only when the phlebotomist has a cut on the hand

Answer: C. OSHA requires gloves when hand contact with blood is reasonably expected and when performing vascular access procedures.

Why not A: Standard precautions treat every patient as potentially infectious.

Why not B: Venipuncture also requires gloves.

Why not D: Gloves are required for every draw, not only with hand injuries.

Exam tactic: Every draw, gloves on.

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More about the NHA CPT

PassPulse is an independent exam preparation tool. We are not affiliated with, endorsed by, or sponsored by the National Healthcareer Association (NHA), the American Society for Clinical Pathology (ASCP), the National Center for Competency Testing (NCCT) or American Medical Technologists (AMT). Exam, credential and product names mentioned on this page are trademarks of their respective owners and are used only to identify the exams described.

These practice questions are written by PassPulse to reflect the published exam content outline. They are not actual exam questions. Questions, answers and explanations may contain errors or be out of date, and they are for exam study only, not for clinical or professional practice: always check facts against the certifying body's official materials, current standards and your employer's policies. Scores and projections on this page are estimates based on a small number of questions and do not predict your result on the real exam. Area weights come from the official outline as of the date shown; always confirm current exam details with the certifying body.
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